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Literature summary extracted from

  • Sehanobish, E.; Chacon-Verdu, M.D.; Sanchez-Amat, A.; Davidson, V.L.
    Roles of active site residues in LodA, a cysteine tryptophylquinone dependent epsilon-lysine oxidase (2015), Arch. Biochem. Biophys., 579, 26-32 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.20 expressed in Escherichia coli Rosetta cells Marinomonas mediterranea

Protein Variants

EC Number Protein Variants Comment Organism
1.4.3.20 C448A the mutation causes large increases in the Km values for L-lysine Marinomonas mediterranea
1.4.3.20 C448D the mutation causes large increases in the Km values for L-lysine Marinomonas mediterranea
1.4.3.20 K530A the variant exhibits diminished levels of cysteine tryptophylquinone but significantly increased kcat value. The mutation causes large increases in the Km values for L-lysine Marinomonas mediterranea
1.4.3.20 K530R the variant exhibits diminished levels of cysteine tryptophylquinone but significantly increased kcat value. The mutation causes large increases in the Km values for L-lysine Marinomonas mediterranea
1.4.3.20 Y211A the variant the highest level of cysteine tryptophylquinone but exhibits no activity Marinomonas mediterranea
1.4.3.20 Y211E the variant the highest level of cysteine tryptophylquinone but exhibits no activity Marinomonas mediterranea
1.4.3.20 Y211F the mutant has reduced levels of cysteine tryptophylquinone but near normal levels of kcat. The mutation causes large increases in the Km values for L-lysine Marinomonas mediterranea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.20 0.0039
-
 L-lysine wild type enzyme, at pH 7.5 and 25°C Marinomonas mediterranea
1.4.3.20 0.048
-
 L-lysine mutant enzyme C448A, at pH 7.5 and 25°C Marinomonas mediterranea
1.4.3.20 0.052
-
 L-lysine mutant enzyme K530A, at pH 7.5 and 25°C Marinomonas mediterranea
1.4.3.20 0.113
-
 L-lysine mutant enzyme K530R, at pH 7.5 and 25°C Marinomonas mediterranea
1.4.3.20 0.129
-
 L-lysine mutant enzyme Y211F, at pH 7.5 and 25°C Marinomonas mediterranea
1.4.3.20 0.142
-
 L-lysine mutant enzyme C448D, at pH 7.5 and 25°C Marinomonas mediterranea
1.4.3.20 0.199
-
 O2 wild type enzyme, at pH 7.5 and 25°C Marinomonas mediterranea
1.4.3.20 0.386
-
 O2 mutant enzyme C448D, at pH 7.5 and 25°C Marinomonas mediterranea
1.4.3.20 0.522
-
 O2 mutant enzyme Y211F, at pH 7.5 and 25°C Marinomonas mediterranea
1.4.3.20 0.543
-
 O2 mutant enzyme C448A, at pH 7.5 and 25°C Marinomonas mediterranea
1.4.3.20 0.706
-
 O2 mutant enzyme K530R, at pH 7.5 and 25°C Marinomonas mediterranea
1.4.3.20 1.043
-
 O2 mutant enzyme K530A, at pH 7.5 and 25°C Marinomonas mediterranea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.20 L-lysine + O2 + H2O Marinomonas mediterranea
-
 (S)-2-amino-6-oxohexanoate + H2O2 + NH3
-
 ?
1.4.3.20 L-lysine + O2 + H2O Marinomonas mediterranea NBRC 103028
-
 (S)-2-amino-6-oxohexanoate + H2O2 + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.20 Marinomonas mediterranea F2JXJ3
-
-
1.4.3.20 Marinomonas mediterranea NBRC 103028 F2JXJ3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.20 L-lysine + O2 + H2O
-
 Marinomonas mediterranea (S)-2-amino-6-oxohexanoate + H2O2 + NH3
-
 ?
1.4.3.20 L-lysine + O2 + H2O
-
 Marinomonas mediterranea NBRC 103028 (S)-2-amino-6-oxohexanoate + H2O2 + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.3.20 epsilon-lysine oxidase
-
 Marinomonas mediterranea
1.4.3.20 LodA
-
 Marinomonas mediterranea
1.4.3.20 marinocine
-
 Marinomonas mediterranea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.20 0.19
-
 L-lysine mutant enzyme Y211F, at pH 7.5 and 25°C Marinomonas mediterranea
1.4.3.20 0.29
-
 L-lysine mutant enzyme C448D, at pH 7.5 and 25°C Marinomonas mediterranea
1.4.3.20 0.34
-
 L-lysine wild type enzyme, at pH 7.5 and 25°C Marinomonas mediterranea
1.4.3.20 0.39
-
 L-lysine mutant enzyme C448A, at pH 7.5 and 25°C Marinomonas mediterranea
1.4.3.20 0.68
-
 L-lysine mutant enzyme K530R, at pH 7.5 and 25°C Marinomonas mediterranea
1.4.3.20 1.2
-
 L-lysine mutant enzyme K530A, at pH 7.5 and 25°C Marinomonas mediterranea

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.20 cysteine tryptophylquinone dependent on Marinomonas mediterranea